Dengue Virus detection (Types 1-4), RT-PCR

The CDC DENV-1-4 Real-Time RT-PCR Assay is intended for the diagnosis of of dengue virus serotypes 1, 2, 3 or 4 from viral RNA in serum or plasma collected from patients with signs and symptoms consistent with dengue (mild or severe) during the acute phase.

Portal Ordering Code: DENV PCR
CPT Code(s): 87798

Fee Schedule

Storage and Transport Criteria: Specimen Storage Specimen Transport

Acceptable Specimens

Minimum Volume

Frozen Range
-70°C - 0°C

Refrigerated Range
0.1°C - 8°C

Room Temperature Range
8.1°C - 30°C

Stability at
4°C - 25°C

Serum, Separated plasma (sodium citrate) 1 mL Acceptable - Freeze within 2 hours of collection Reject Reject Transport on dry ice only
Includes: Test distinguishs DENV serotypes 1, 2, 3, and 4.
Methodology: RT-PCR
Assay Category: FDA Approved
Reference Range: See laboratory report
Alternative Names: CDC DENV-1-4

Real-Time RT-PCR Assay for Detection and Serotype Identification of Dengue Virus

Break bone fever
Preferred Specimen(s): Serum

Separated plasma (sodium citrate)
Alternative Specimen(s): N/A
Minimum Volume: 1 mL
Special Instructions: N/A
Reject Criteria: Inadequately labeled specimens

Leaking specimens

QNS (quantities not sufficient)

Specimens not meeting the Storage & Transport criteria upon receipt at laboratory
Setup Schedule: Testing is performed on an as-needed basis.
Turn around time (TAT): 2 business days from arrival at the lab

Acceptable Specimens	Minimum Volume	Frozen Range -70°C - 0°C	Refrigerated Range 0.1°C - 8°C	Room Temperature Range 8.1°C - 30°C	Stability at 4°C - 25°C
		Specimen Storage			Specimen Transport
Serum, Separated plasma (sodium citrate)	1 mL	Acceptable - Freeze within 2 hours of collection	Reject	Reject	Transport on dry ice only